大腸桿菌自誘導培養基適用于由功能性的lac操縱子(包括lac Y和lac Z)控制的表達系統，如PET系列。表達菌種可用BL21(DE3)。大腸桿菌自誘導培養基(E.coli Auto-Induction Medium)是在LB培養基的基礎上進(jìn)行改進(jìn)優(yōu)化配方，可用于lac調控表達的大腸桿菌菌株培養及蛋白表達。含有多種營(yíng)養物質(zhì)并額外添加微量元素、氨基酸等，碳源梯度設計既能滿(mǎn)足菌體生長(cháng)需求，同時(shí)能實(shí)現外源蛋白在細菌達到飽和生長(cháng)期后的自動(dòng)誘發(fā)。同等產(chǎn)量相比傳統LB使用更少培養體積，無(wú)需密切監控菌液密度的過(guò)程(測OD)，且無(wú)需額外添加IPTG。本品同樣適用于多克隆表達，高通量選。產(chǎn)品為粉末形式，500g/瓶，按實(shí)驗室已經(jīng)習慣的常規LB培養基樣配制，配制方便。

LB自誘導培養基LB Broth (Auto Induction Medium)產(chǎn)品介紹選購指南

用于制備不含微量元素的LB培養基的固體粉末，添加有葡萄糖和α-乳糖，以便在大腸桿菌中通過(guò)IPTG誘導的啟動(dòng)子自動(dòng)誘導蛋白表達。

Quantity: 500g

Appearance: Beige powder. Autoclaved medium should be amber.

Storage: 2oC – 25oC. When not in use, keep container closed to avoid hydration.

Preparation:
Add 34,85g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121oC for 15 to 20 minutes. Store at 2oC to 8oC.

Formulation (g/L)
Tryptone: 10,00
Yeast Extract: 5,00
MgSO4: 0,15
(NH4) 2SO4: 3,30
KH2PO4: 6,80
Na2HPO4: 7,10
Glucose 0,50
Alpha Lactose 2,00
Final pH (25oC): 7,0 ± 0,2